花生学报2012，Vol.41Issue(4)：1-7,7.
花生愈伤组织的诱导及细胞悬浮培养体系的建立
Callus Induction and Establishment of Cell Suspension Culture System for Peanut
摘要
Abstract
With Zhonghua2 as material, the optimum culture conditions of callus induction and cell suspension culture were studied, the growth characteristics of suspension cell were detected, and a cell suspension culture system was established. The results showed that leaf was the best explant for callus induction and cell suspension culture, the optimal medium for callus induction was MS-+- 6-BA 2.0 mg/L+2,4-D 2.0 mg/L, the optimal formula for suspension culture was MS+6-BA 2.0 rag/L+ 2,4-D 2. 0 mg/L + KT 1. 5 mg/L. The growth of suspension cells exhibited an S-shaped curve, growth mass reached the maximum after 12d culture, the optimal subculture cycle was around 12d. In the process of suspension culture, the suspension cell number exhibited the change trend: firstly increased, then decreased. The change trends of pH value and conductivity showed the same: firstly decreased, then increased, and then became stable.关键词
花生/愈伤组织/悬浮培养Key words
peanut/callus/suspension culture分类
农业科技引用本文复制引用
黄文静,许春燕,何虎翼,邓伦武,李创珍,韦善清,何龙飞..花生愈伤组织的诱导及细胞悬浮培养体系的建立[J].花生学报,2012,41(4):1-7,7.基金项目
国家自然科学基金资助项目 （）
