菌物研究2012，Vol.10Issue(4)：250-254,5.
鹅膏菌抑真菌活性及其活性物质的提取工艺优化（英文）
Anti-Fungal Effect of Amanita sp. and Optimized Extraction Technology of Its Active Substances
摘要
Abstract
The anti-fungal effects of Amanita sp. on Cytospora chrysosperma were analyzed by detecting the growth rates of mycelia and spore germinated on medium with active substances. The cultured fluid and mycelium of cultured suspension of Amanita sp. were separated after being cultured 15 d. The in- hibiting effect of cultured fluid, mycelium extractions and cuhured suspension were tested severally on Cytospora chrysosperma. The results showed that the growth-inhibiting rate of the cultured suspension on Cytospora chrysosperma was 68.90% and was the best among the 3 samples. Not only the cultured sus- pension, the cultured fluid and mycelium had evident inhibiting effects on the pathogenic spores in which spores had not germinated in 10 days. The mycelia were extracted by alcohol, acetone, n-butyl alcohol and ethyl acetate, and the inhibiting effect on Cytospora chrysosperma was as follows : all of mycelium ex- tractions by alcohol and acetone had evident inhibiting effects on mycelium growth and spore germination, which had significant differences (a = 0.01) with the control, but the mycelium extraction of n-butyl al- cohol had the lowest growth-inhibiting effect on Cytospora chrysosperma and had no significant difference ( a = 0.01 ) with the control. The extraction technology of active substances optimized by the inhibiting effect on Cytospora chrysosperma was as follows: shake culture was the best culture way; alcohol was the optimal extracting solvents; ultrasonic, dip and heat all can be used for extracting inhibiting active sub- stances and they had no significant difference among one another.关键词
鹅膏菌/金黄壳囊孢菌/提取工艺/抑真菌活性Key words
Amanita sp./Cytospora chrysosperma/extraction technology/anti-fungal effect分类
农业科技引用本文复制引用
冀瑞卿,李玉,宋瑞清,孟黎鹏..鹅膏菌抑真菌活性及其活性物质的提取工艺优化（英文）[J].菌物研究,2012,10(4):250-254,5.基金项目
Jilin Agricultural University Dr.Foundation （）
National Natural Science Foundation of China （）
International Cooperate Item of Heilongjiang Province （）
