生物灾害科学Issue(4)：347-351,5.
PTD-Cry3Aa原核融合表达产物对甘薯小象甲的活性研究
A Study on the Activity of the Prokaryotic Expression Products of PTDCry3Aa Fusion Gene to Sweet Potato Weevil,Cylas formicarius
摘要
Abstract
In order to study the synergism of the TAT PTD(the protein transduction domain in HIV1 transactivator of transcription protein) to the Cry3Aa from Bacillus thuringiensis,the fusion gene PTDCry3Aa and gene Cry3Aa were expressed in E.coli.cells.The toxicity of prokaryotic expression products from PTDCry3Aa and Cry3Aa was tested in sweet potato weevil,Cylas formicarius.The bioassay results showed that the primary extracts of PTDCry3Aa and Cry3Aa inclusion bodies exhibited activities against the adults and larvae of sweet potato weevil.The results also indicated there was a synergism of PTD to Cry3Aa both on the adults and the larvae.The synergistic rates were 2.01 folds for the adults while 3.19 folds for the larvae.The study results would play an important role in biological control and transgenic sweet potato against sweet potato weevil.关键词
Cry3Aa/蛋白转导结构域（PTD）/甘薯小象甲Key words
Cry3Aa/TAT protein transduction domain/Cylas formicariu分类
农业科技引用本文复制引用
邱道寿,刘晓津..PTD-Cry3Aa原核融合表达产物对甘薯小象甲的活性研究[J].生物灾害科学,2012,(4):347-351,5.基金项目
广东省自然科学基金项目 （）
