乳业科学与技术2012，Vol.35Issue(6)：23-25,3.
快速测定婴幼儿乳粉中的氯霉素残留
Rapid Determination of Residual Chloramphenicol in Infant Milk Powder by LC-MS-MS
王多娇 1颜春荣 1曲斌 2卢剑 1徐春祥1
作者信息
- 1. 江苏省产品质量监督检验研究院食品检测中心,江苏南京210029
- 2. 江苏省畜产品质量检验测试中心,江苏南京210036
- 折叠
摘要
Abstract
A high performance liquid chromatography tandem mass spectrometric (HPLC-MS-MS) method was developed for rapid determination of chloramphenicol residue in infant milk powder. DS-Chloramphenicol was used as internal standard. Samples were alkalified, extracted with ethyl acetate and centrifuged at a low temperature. Afterwards, the supernatant was concentrated by nitrogen gas blowing and dissolved again in HPLC mobile phase before HPLC-MS- MS analysis. An excellent linear relationship between peak area and chloramphenicol concentration was obtained over the concentration range of 0.1 to 5 μg/L. Average recoveries of chloramphenicol in a blank matrix across three spike levels ranged from 80% to 110%, and inter-batch and intra-batch precision (RSD) was less than 15%. The limit of quantification of this method was 0.1 μg/kg. This method was rapid, simple and suitable for rapid determination of chloramphenicol residue in infant milk powder.关键词
婴幼儿乳粉/氯霉素/残留/LC—MS—MSKey words
infant milkpowder/chloramphenicol/residue/LC-MS-MS分类
化学化工引用本文复制引用
王多娇,颜春荣,曲斌,卢剑,徐春祥..快速测定婴幼儿乳粉中的氯霉素残留[J].乳业科学与技术,2012,35(6):23-25,3.
