应用荧光光谱法研究污泥活性变化OACSTPCD
Study of Sludge Activity by Fluorescence Spectroscopy
本研究以LIVE/DEAD Baclight染色法测定活性污泥中的活菌水平以表征其活性,采用荧光染色剂SYTO!9和碘化丙啶（PI）对活性污泥进行染色,并利用日立F-7000荧光分光光度计测量其绿色荧光（Fg,波长500—510 nm）强度来表征活性污泥中活菌水平。该法直接对稀释后的污泥悬浊液进行测量,简化了测量步骤。通过纯菌E.coli的LIVE/DEAD染色试验,发现活菌比例与绿色/红色荧光的比值存在较高的相关性,相关系数R2=0.982。通…查看全部>>
ludge activity was characterized by the live bacteria ratio in this study via LIVE/DEAD Baclight dyed method combined with fluorescence spectroscopy.The sludge samples were stained by SYTO 9 and PI(propidium iodide),then analyzed by F-7000 fluorospectrophotometer.The density of green fluorescence was used to indicate the level of live bacteria in the samples stained by SYTO 9 and PI.The competitive advantages of this method was proved including conveni…查看全部>>
谢恩;罗固源
重庆大学三峡库区生态环境教育部重点实验室,重庆400045重庆大学三峡库区生态环境教育部重点实验室,重庆400045
资源环境
荧光光谱法LIVE/DEAD活性污泥
Fluorescence spectrometryLIVE/DEADActivated sludge
《现代科学仪器》 2012 (6)
134-136,3
国际科技合作项目（2007DFA90660）
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