现代科学仪器Issue(6)：134-136,3.
应用荧光光谱法研究污泥活性变化
Study of Sludge Activity by Fluorescence Spectroscopy
摘要
Abstract
ludge activity was characterized by the live bacteria ratio in this study via LIVE/DEAD Baclight dyed method combined with fluorescence spectroscopy.The sludge samples were stained by SYTO 9 and PI(propidium iodide),then analyzed by F-7000 fluorospectrophotometer.The density of green fluorescence was used to indicate the level of live bacteria in the samples stained by SYTO 9 and PI.The competitive advantages of this method was proved including convenient and easy.The correlation coefficient between live bacteria ratio and the ratio of green/red fluorescence intensity(G/R) has been proved,it was 0.982 via LIVE/DEAD Baclight dyed experiment by used escherichia coli(E.coli).After bacterial decay experiment,the G/R in 485nm decreased from 3.69 to 1.74,the live bacteria ratio declined by 52.85%,it’s close to the result which gray-scale analyzed by software.关键词
荧光光谱法/LIVE/DEAD/活性污泥Key words
Fluorescence spectrometry/LIVE/DEAD/Activated sludge分类
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谢恩,罗固源..应用荧光光谱法研究污泥活性变化[J].现代科学仪器,2012,(6):134-136,3.基金项目
国际科技合作项目 （）
