中国兽医科学2012，Vol.42Issue(12)：1224-1229,6.
猪传染性胃肠炎病毒四川株的分离鉴定与一步生长曲线的测定
Isolation and identification of porcine transmissible gastroenteritis virus in Sichuan Province and determination of one-step growth curve
摘要
Abstract
To study the growth dynamics of porcine transmissible gastroenteritis virus(TGEV),a strain of virus(SC-T) was isolated from diarrhea piglets samples in Sichuan by cultivation in PK-15 cells,and the isolated virus was proved to be TGEV through PCR detection,the physical and chemical properties experiment,and microneutralization test.The SC-T virus was used to infect PK-15 cells,and the infected cells and supernatant were collected at different time points post-infection.Fluorescent quantitative PCR was used to analyse TGEV RNA quantity in different samples,and the one-step growth curve of SC-T was determined according to the results.In result,at hour 2-8 post-infection,TGEV RNA increase rapidly in cells,and at hour 8-32 post-infection,virus RNA maintained at a high level.TGEV RNA in supernatant was in an S shaped growth curve.At 0-6 h post-infection,there was a period of incubation,and virus RNA maintained at a low level.At 6-28 h post-infection,there was a stage of breakthrough,and virus RNA was undertaken a logarithmic growth.At hour 28-32 post-infection,the growth rate slowed down,and virus RNA maintained at a high level and stepped into stationary phase gradually.In conclusion,virion was released from the infected cells while TGEV multiplied to a certain number in TGEV-infected PK-15 cells.关键词
猪传染性胃肠炎病毒/分离鉴定/荧光定量PCR/一步生长曲线Key words
transmissible gastroenteritis virus/isolation and identification/fluorescent quantitativePCR/one-step growth curve分类
农业科技引用本文复制引用
代洪波,陈蕾,朱玲,周远成,郭万柱,徐志文..猪传染性胃肠炎病毒四川株的分离鉴定与一步生长曲线的测定[J].中国兽医科学,2012,42(12):1224-1229,6.基金项目
四川省科技支撑计划项目 （）
四川省科技支撑计划项目一生猪现代产业链关键技术研究集成与产业化示范 （）
