中国兽医科学2012，Vol.42Issue(12)：1230-1236,7.
副猪嗜血杆菌外膜蛋白P5的抗原特性研究
Study on antigenic characterization of outer membrane protein P5 of Haemophilus parasuis
摘要
Abstract
The aim of this study was to learn antigenic characterization of the outer membrane protein P5(OmpP5) of Haemophilus parasuis.OmpP5 gene was cloned from H.parasuis servoar 5 and expressed in Escherichia coli.The cross-reaction of OmpP5 in different serotype of H.parasuis and with related gram-positive bacteria was analyzed by Western-blot and ELISA.The amino acid sequence identity and the phylogenetic analysis between these strains were analyzed.Lastly,the immunoprotection of OmpP5 was evaluated by a lethal challenge dose of H.parasuis serovar 5 in mice.As a result,recombinant OmpP5 could react with the antibodies of all the 15 serovars reference strains of H.parasuis,and it also react with antibodies of some other gram-negative and gram-positive bacteria,including Haemophilus influenzae,Acdnobacillus pleuropneumoniae,Salmonella,Pasteurella multocida,Shigella and Listeria monocytogenes.The bioinformatics analysis showed that the amino acid sequences identities of the 15 serovars of H.parasuis were from 88.4% to 100% and they shared from 45.9% to 72.7% with the cross-reaction bacteria.This demonstrated that the H.parasuis OmpP5 is highly conserved in the different serotype of H.parasuis and also conserved in the different kind of gram-positive bacteria.In addition,this study demonstrated that whilst all the unimmunized mice as control died by 16 hour post-challenge,two mice(out of 7) from the immunized groups survived beyond 7 days post-challenge.These result showed that the recombinant OmpP5 could excite strong antibody response and provide partially immunoprotection.关键词
副猪嗜血杆菌/外膜蛋白P5/交叉反应性/免疫保护Key words
Haemophilus parasuis/OmpP5/Cross-reaction/Immunoprotection分类
农业科技引用本文复制引用
张斌,海泉,汤承,邵国青,岳华..副猪嗜血杆菌外膜蛋白P5的抗原特性研究[J].中国兽医科学,2012,42(12):1230-1236,7.基金项目
“十二五”国家高技术研究发展（863）计划项目 （）
