中国兽医科学2012，Vol.42Issue(12)：1264-1267,4.
猪环曲病毒RT-PCR检测方法的建立及其应用
Development and application of RT-PCR assay for detection of porcine torovirus
摘要
Abstract
In order to establish a rapid RT-PCR assay for detection of porcine torovirus,a pair of special primers was designed according to the published sequences of porcine torovirus in GenBank.In the specificity test,we used this method to amplify pseudorabies virus,porcine kobuvirus,TGEV,PEDV,PRoV,CSFV,Streptococcus suis,and Escherichia coli.The results indicated that only porcine torovirus was successfully amplified a target fragment of 451 bp in size.When the sequence from the PCR products were compared to the sequence from GenBank(GenBank accession number:AJ575372),they showed 93% identity.Testing of the sensitivity of RT-PCR indicated that the lowest detection limit was 10 pg nuclear acids.Forty three stool specimens collected from different farms in Sichuan Province were detected by the established method,and the positive rate was 25.6%.The results showed that the RT-PCR was specific and sensitive to detect porcine torovirus,which can apply for the clinic detection and molecular epidemiological survey.关键词
猪环曲病毒/RT-PCR/检测Key words
porcine torovirus/RT-PCR/detection分类
农业科技引用本文复制引用
周璐,李淞,徐薇薇,周远成,赵睿,蔡雨函,吴江江,朱玲,徐志文,郭万柱..猪环曲病毒RT-PCR检测方法的建立及其应用[J].中国兽医科学,2012,42(12):1264-1267,4.基金项目
四川省科技支撑计划项目 （）
