中国兽医科学2012，Vol.42Issue(12)：1268-1272,5.
猪肺炎支原体P97 TaqMan-BHQ荧光定量PCR检测方法的建立及应用
Development and application of TaqMan-BHQ real time PCR assay for detection of Mycoplasma hyopneumoniae P97
摘要
Abstract
A pair of primers and TaqMan-BHQ probe were designed according to the Mycoplasma hyopneumoniae(Mhp) P97 sequence,and TaqMan-BHQ real time PCR assay was successfully established for detection of Mhp.In result,the developed method could specifically detect Mhp,and had no cross-reaction with pathogens of other swine diseases.There was an excellent linear correlation during the DNA concentration from 101 copies/μL to 107 copies/μL.The analytic sensitivity of the assay was 10 copies/ μL.The equation of the assay was Ct=-3.426×lg(conc)+43.343,the correlation coefficient of the standard curve was 0.997,and amplification efficiency was 95.85% with good repeatability.The 48 pig lung tissues from pig farms in Jiangsu Province were detected,and the positive rates were 89.58%.The result showed that the TaqMan-BHQ real time PCR can be useful for rapid detection of Mhp in clinic detection and for epidemic investigation of mycoplasmal pneumonia of swine in field study.关键词
猪肺炎支原体/P97基因/荧光定量PCR/TaqMan-BHQ探针Key words
Mycoplasma hyopneumoniae/P97 gene/real time PCR/TaqMan-BHQ probe分类
农业科技引用本文复制引用
武昱孜,靳蒙蒙,白方方,张旭,华利忠,雷治海,邵国青..猪肺炎支原体P97 TaqMan-BHQ荧光定量PCR检测方法的建立及应用[J].中国兽医科学,2012,42(12):1268-1272,5.基金项目
江苏省农业自主创新基金项目[CX（11）2059] （）
国家自然科学基金项目 （）
