中南民族大学学报：自然科学版2012，Vol.31Issue(4)：36-40,5.
雪上一枝蒿醇提物体外抗炎作用的研究
Investigation on the Anti-Inflammatory Effect of Ethanolic Extract of Aconitum brachypodum Diels in vitro
摘要
Abstract
The effect of extracts from Aconitum brachypodum Diels (EABD) on the mouse peritoneal macrophage activity and apoptosis was studied. The released nitric oxide (NO) and reactive oxygen species (ROS) was induced by Lipopolysaccharide (LPS) in vitro, and the macrophages were treated with various concentrations of EABD after LPS indction. The activities and apoptosis of macrophages were detected by MTT assay and Hoechst 33258 fluorescent staining. ROS and NO production were determined by DCFH-DA Fluorescence probe and Griess Reagent. The results indicated that EABD could inhibit the damage and apoptosis of mouse peritoneal macrophages induced by LPS. 20,100 μg/mL EABD could decreas ROS and NO production in a dose-dependent manner. This demonstrated that EABD may play the antiinflammatory role through anti-oxidative pathway in vitro.关键词
雪上一枝蒿/凋亡/一氧化氮/活性氧Key words
Aconitum brachypodum diel/apoptosis/NO/ROS分类
医药卫生引用本文复制引用
黄先菊,任炜,潘乐,陈芙蓉,邹大江..雪上一枝蒿醇提物体外抗炎作用的研究[J].中南民族大学学报：自然科学版,2012,31(4):36-40,5.基金项目
湖北省高等学校优秀中青年科技创新团队计划资助项目 （）
中央高校基本科研业务费专项资金资助项目 （）
