安徽农业科学Issue(2)：500-502,3.
三元复合驱采油污水对水丝蚓DNA损伤的影响
DNA Damage of Limnodrilu hoffmeisteri Somatopiasm by Oily Sewage of Alkali-surfactant-polymer Flooding
摘要
Abstract
[Objective] The aim was to study the genotoxicity effects of oily sewage of alkali-surfactant-polymer flooding on aquatic animals. [Method] Limnodrilu hoffmeisteri exposed to oily sewage with concentrations of 12% ,36% ,24% and 44% ( V/V) respectively. Then the lysed cells were detected by single cell gel electrophoresis under alkaline conditions and the DNA damage of limnodnlu hoffmeisteri cells was detected by using the tail length (TL) ,Head DNA content and tail DNA content as the indexes. [Result] The oily sewage of alkali-surfactant-polymer flooding could cause the damage of Limnodrilu hoffmeisteri somatopiasm DNA. The tail length and tail DNA content of all the treated groups increased significantly compared with those of control (P <0.05) ,and the head DNA content were decreased significantly (P <0.05). The DNA damage showed obvious "dose-effect" relationship along with the concentration increased of oily sewage. [ Conclusion] This study showed that the oily sewage of alkali-surfactant-polymer flooding has genotoxicity effect on Limnodrilu hoffmeisteri,and may have a serious impact on the ecological environment of oil field.关键词
三元复合驱/霍普水丝蚓/急性毒性/DNA损伤Key words
Alkali-surfactant-polymer flooding/ Limnodrilu hoffmeisteri/ Acute toxicity/ DNA damage分类
农业科技引用本文复制引用
尹伟平,徐长君,殷亚杰,秦姝冕,李铭,聂春雨..三元复合驱采油污水对水丝蚓DNA损伤的影响[J].安徽农业科学,2013,(2):500-502,3.基金项目
黑龙江省自然科学基金项目(C200827). （C200827）
