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健康仔猪肠道内乳酸菌的分离与鉴定

顾甜甜 秦晓冰 单虎 温建新 周保琨

安徽农业科学Issue(2):630-632,3.
安徽农业科学Issue(2):630-632,3.

健康仔猪肠道内乳酸菌的分离与鉴定

Separation and Identification of Lactobacillus from the Intestinal Tract of Healthy Piglet

顾甜甜 1秦晓冰 1单虎 1温建新 1周保琨2

作者信息

  • 1. 青岛农业大学动物科技学院,山东青岛266109
  • 2. 山东省即墨市畜牧兽医局,山东即墨266200
  • 折叠

摘要

Abstract

[Objective] Lactobacillus were separated and identified from intestinal tract of healthy piglet. [Method] The Lactobacillus were i-dentified through bacteria morphology, physiological and biochemical tests and 16S rRNA sequences analysis. [Result] 9 strains of 10 suspected Lactobacillus are Lactobacillales, and Cl, C2, C3 belong to Streptococcus, C2, F2, F3 are Lactobacillus johnsonii, C4, F4 are Enterococcus fae-cium and C5 is Lactobacillus acidophilus. [ Conclusion ] The study provides a bacteria strain foundation for preparation of piglet probiotics.

关键词

乳酸菌/分离/鉴定/生化试验/16S rRNA序列分析

Key words

Lactobacillus/ Separation/ Identification/ Biochemical testing/16S rRNA sequences analysis

分类

农业科技

引用本文复制引用

顾甜甜,秦晓冰,单虎,温建新,周保琨..健康仔猪肠道内乳酸菌的分离与鉴定[J].安徽农业科学,2013,(2):630-632,3.

基金项目

山东省优秀中青年科学家科研奖励基金(BS2011SW010). (BS2011SW010)

安徽农业科学

0517-6611

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