安徽农业科学Issue(2):635-637,3.
猪发育期NPY-Y1和GPR54受体基因的表达规律研究
Study on the Regularity of NPY-Y1, GPR54 Acceptor Gene Development Expression in Development Stage of Pig
摘要
Abstract
[ Objective] To study development expression of NPY-Y1 and GPR54 acceptor gene in development stage of pig. f Method] Three female Sujiang pigs were selected in different stages of birth, 60, 120, puberty and 180 days, total RNA extracted from tissue samples of hypothal-amus, pituitary and ovaries, development expression of NPY- Y1 and GPR54 gene were determined by quantitative RT-PCR. [ Result ] The results showed that the expression abundance of NPY-Yl mRNA was gradually descending from birth to puberty, and after puberty the expression abundance was ascending tendency in all three tissues, and the expression abundance at the stage of puberty was significant difference with the other stage(P <0.05). But the expression abundance of GPR54 mRNA was gradually ascending from birth to puberty, and after puberty the expression abundance was descending tendency in all three tissues, the expression abundance at the stage of puberty was also significant difference with the other stage(P <0.05). [Conclusion] The expression abundance of GPR54 mRNA was associativity with NPY-Y1 mRNA in development stage of pig.关键词
猪/NPy-Y1基因/GPR54基因/发育性变化Key words
Pig/ NPY-Yl gene/ GPR54 gene/ Development expression分类
农业科技引用本文复制引用
周春宝,倪黎纲,丁家桐,韩大勇,朱道仙..猪发育期NPY-Y1和GPR54受体基因的表达规律研究[J].安徽农业科学,2013,(2):635-637,3.基金项目
江苏省自然科学基金项目(BK2005050) (BK2005050)
江苏省农业三项工程项目(sx(2007)088). (sx(2007)