安徽农业科学Issue(2)：640-641,700,3.
A型口蹄疫病毒A/HeN/1/2009株全基因组序列的测定及其基因特征分析
The Whole Genome Sequencing and Characteristics Analysis of FMDV Serotype A/HeN/1/2009
摘要
Abstract
[Objective] The study was to sequence the whole genome of FMDV serotype A/HeN/1/2009 and analyze the characteristics contained in these gene sequences, this will be help us to study the pathogenicity and epidemicity of FMDV serotype A frequently occurred in these years and also be a great help to develop vaccines to prevent and control FMD. [Method] The related gene segments of A/HeN/1/2009 were amplified by RT-PCR and these genes were sent to sequencing company for sequencing. The epidemiological features of this serotype were analyzed by DNAStar. [ Result ] The whole genome was 8 171 nts [ not including poly (C) section and poly ( A) tail ]. The length of 5 -UTR ,3 -UTR and protein coding regions were 1 080 nts,92 nts,and 6 999 nts respectively. The analysis of epidemiological features showed that A/HeN/1/2009 was the VN09 subline belong to southeast Asia topology type Laos03 system,the homologies in turn were 87.3% -91.8% ,93.7% -94.5% ,96.9% -98.4%. [Conclusion] A24V,N85R,S196T of VP1 protein, 161 V,T128S,E147G and A134V of 3A protein played key role in the pathogenicity of FMDV serotype A. We also found that the motif sequence of VP1 in A/HeN/1/2009 was RSD.关键词
口蹄疫病毒/A/HeN/1/2009株/全基因组/东南亚拓扑型Key words
FMDV/ A/HeN/1/2009/ Whole genome/ Southeast Asia topological type分类
农业科技引用本文复制引用
谢毅,牟一娇,朱琳,刘强,赵兴绪..A型口蹄疫病毒A/HeN/1/2009株全基因组序列的测定及其基因特征分析[J].安徽农业科学,2013,(2):640-641,700,3.基金项目
科技部国际合作重点项目(2005DFA30720). （2005DFA30720）
