北京林业大学学报2013，Vol.35Issue(2)：63-68,6.
黑土区不同林龄落叶松人工林土壤微生物群落功能多样性的对比研究
Comparative studies on functional diversity of soil microbial community of larch plantations with different ages in black soil region, northeastern China
摘要
Abstract
Metabolism diversity of soil microbial carbon sources of surface soil (0-20 cm) from 21-, 30-, 40-, and 52-year-old larch (Larix gmelini) plantations in typical black soil region of northeastern China was studied by using the method of Biolog-ECO microplate culture. Results showed that the average well color development (AWCD) increased gradually with forest age increasing, varying from 0.41 to 1.40 and 0.20 to 0. 69 (cultured for 168 hrs) for 0-10 cm and 10-20 cm soil depth, respectively. The peak value of microbial functional diversity was found in 52-year-old plantations, followed by 40-, 30-, 21-year-old in consequence. The microbial functional diversity indices in shallower soil depth all showed higher values; for soil in 0-10 cm and 10-20 cm depth, richness index was 12-25 and 6-14, Shannon-Weiner diversity index was 2. 55-3. 12 and 1. 83-2. 62, Simpson diversity index was 0. 89-0. 95 and 0. 76-0. 91 , Mclntosh index was 4. 22-9. 49 and 2. 52-6. 18 , respectively. The utilization efficiency of six carbon sources by the soil microbial community also increased when plantations grew from 21 to 52-year-old. Sensitive carbon sources to cause microbial metabolic differences were carbohydrates, polymers and amines for soil of 0-10 cm, and carboxylic acids in addition to carbohydrates and amines for 10-20 cm also. The significant relationships were observed between the metabolic diversity of microbial communities and soil physical and chemical properties. We conclude that functional diversity of soil microbial community in larch plantations increases gradually with the increase of forest age, and soil microbial functional diversity can be improved by implementing plantation vegetations recovery. The results presented in this paper could further suggest a reference for revealing transmutation of soil quality under plantations of black soil region, northeastern China.关键词
落叶松人工林/林龄/黑土区/土壤微生物群落/功能多样性Key words
larch plantations/forest age/black soil region/soil microbial community/functional diversity分类
农业科技引用本文复制引用
范瑞英,杨小燕,王恩姮,邹莉,陈祥伟..黑土区不同林龄落叶松人工林土壤微生物群落功能多样性的对比研究[J].北京林业大学学报,2013,35(2):63-68,6.基金项目
国家自然科学基金项目(41271293). （41271293）
