北京林业大学学报2013，Vol.35Issue(2)：75-79,5.
小蠹科昆虫基因组DNA提取方法的比较研究
Comparison of four genomic DNA extraction methods with Scolytidae beetles
摘要
Abstract
Four methods used for extracting the genomic DNA of bark beetles, Xyleborus mutilatus (Coleoptera, Scolytidae) were studied in this article. An individual X. mutilatus beetle, a bark beetle leg and the specimen reserved in 75% ethanol were selected as testing sample respectively, the genomic DNA of bark beetle was extracted by using four different methods, including classical phenol/chloroform extracting method, improved cetyltrimethyl ammonium bromide ( CTAB) method, the sodium dodecyl sulfate ( SDS) method and animal tissue/cell genomic DNA extraction kit ( i. e. paramagnetic particle method). The extracted genomic DNA was treated with photometric analysis and CO I gene amplification. The results showed that all the four methods can be used for the total DNA extraction. For the specimen soaked in 75% ethanol, these four methods were all applicable, however, only the paramagnetic particle method was suitable for the DNA extraction of the specimen, which had been soaked in 75% ethanol over two years. Furthermore, the paramagnetic particle method was also applicable for the total DNA extraction from microscale tissue. The study provids primary information for further molecular systematic research of bark beetles.关键词
小蠹科/基因组DNA/CO Ⅰ/分子鉴定Key words
Scolytidae/ genomic DNA/ CO I / molecular identification分类
农业科技引用本文复制引用
常虹,郝德君,杨晓军,肖荣堂,刘勇,钱路,安榆林..小蠹科昆虫基因组DNA提取方法的比较研究[J].北京林业大学学报,2013,35(2):75-79,5.基金项目
科技部国际科技合作项目(2009DFA31950)、国家质检总局项目(2010IK256)、国家科技支撑计划课题(2012BAK11B03). （2009DFA31950）
