硅酸盐通报2013，Vol.32Issue(2)：200-204,5.
纳米银的制备、修饰及与鲱鱼精DNA的作用
Preparation and Modification of Nanosilver and the Interaction between it and Herring Sperm DNA
摘要
Abstract
The nanosilver was prepared by redox and the SiO2 layer successfully coated on the surface of silver nanoparticles in ethanol dissolvent, obtained Ag-SiO2 core-shell structure (Ag@ SiO2, Ag core, SiO2 shell), The silver nanoparticles and Ag@ SiO2 structure were characterized by transmission electron microscope ( TEM ) , Uv-vis absorption spectrum ( Uv-vis ) , fluorescence spectrum respectively. The experiments show that the formation of Ag@ SiO2 is affected by the order of ammonia addition. The SiO2 shell thickness plays a notable role in spectral properties. The maximum absorption peak of Ag core is red-shifed and intensity is declined after the coverage of SiO2 shell. But the maximum absorption peak of Ag core is blue-shifed and intensity is increased when the thickness of SiO2 shell is more than 50 nm in this experiment. The emission spectrum indicates that the fluorescence intensity of nanosilver core is reduced by SiO2 shell. Moreover, the interaction between Ag@ SiO2 and herring sperm DNA was investigated by spectrometry. The results display that the absorption spectrum of herring sperm DNA and the emission spectrum of Ag @ SiO2 do not apparently change. Ag @ SiO2 nanoparticles have good biocompatibility, which provides an important basis for the safety of Ag@ SiO2 nanoparticles, both toxicology and biomedicine aspects.关键词
纳米银/Ag@SiO2核壳结构/鲱鱼精DNA/光谱Key words
silver nanoparticles/ Ag@ SiO2 core-shell structure/ herring sperm DNA/ spectrum分类
医药卫生引用本文复制引用
张怀斌,李怀祥,马丽英,荣先国,朱崇艳..纳米银的制备、修饰及与鲱鱼精DNA的作用[J].硅酸盐通报,2013,32(2):200-204,5.基金项目
国家自然科学基金资助项目(No.60671010),山东省高等学校科技计划资助项目(J12LD55),滨州医学院科研专项资助项目(BY2009KJ30),滨州医学院大学生科技创新项目(BY2011DKCX13) （No.60671010）
