河南农业科学2013，Vol.42Issue(1)：18-22,5.
光温敏雄性不育玉米9417雄穗幼穗期叶片叶绿体蛋白质差异分析
Differential Analysis of Leaf Chloroplast Protein in Young Spike of Thermo-photo-sensitive Male Sterile Maize Line 9417
郝勇锋 1李维平 1王帅 1姚伟 1周丹平1
作者信息
- 1. 西北农林科技大学生命科学学院,陕西杨凌712100
- 折叠
摘要
Abstract
Taking thermo-photo-sensitive male sterile maize line 9417 as material,the chloroplast proteins in young leaves of male infertile and fertile plants were isolated and analyzed by means of SDS-PAGE,MS and bio-informatics technology. SDS-PAGE showed that there were at least four different bands between male sterile and fertile plants for chloroplast proteins at the sensitive period of young ear development,among which No. 1 and No. 2 bands presented in fertile plants, while No. 3 and No. 4 bands were expressed in sterile plants. Four proteins were identified by MS:AOL_s00004g466,luc7-like protein 3-like, putative T3SS effector EspN2-2 and ABC transporter ATP-binding protein. In fertile plants,No. 1 was a hypothetical protein, while Ul-snRNP component of No. 2 had the function to mediate itself to associate with cap-binding complex in RNA procession and modification. In sterile plants,No. 3 protein had T3SS effect,and No. 4 protein had the effect to transport unidirectionally the substrates in the ATP-binding region of transmembrane. The difference of chloroplast proteins might be related to the male sterility and infertility.关键词
玉米/光温敏雄性不育/叶绿体/蛋白质组学Key words
maize/thermo-photo-sensitive male sterility/chloroplast/proteomics分类
农业科技引用本文复制引用
郝勇锋,李维平,王帅,姚伟,周丹平..光温敏雄性不育玉米9417雄穗幼穗期叶片叶绿体蛋白质差异分析[J].河南农业科学,2013,42(1):18-22,5.
