化学工程2013，Vol.41Issue(2)：6-8,3.DOI:10.3969/j.issn.1005-9954.2013.02.002
不同产地不同部位巫山淫羊藿中黄酮的HPLC分析
HPLC analysis of different parts in Epimedium Wushanense from different regions
周颖欣 1张海荣 2范代娣1
作者信息
- 1. 西北大学化工学院陕西省生物材料与发酵工程技术研究中心陕西省可降解生物医用材料重点实验室,陕西西安710069
- 2. 华陆工程科技有限责任公司,陕西西安710065
- 折叠
摘要
Abstract
The HPLC gradient elution method was established to determine the mass fraction of icariside I , epimedin C and icariin in Epimedium Wushanense at the same time. The phenomenex C18 chromatographic column (5 μm,4. 6 mm ×250 mm) was used. The mobile phases consisted of water (A) and acetonitrile (B). The flow rate was 1.0 mL/min,detecting at 270 nm and 35 ℃. The gradient elution conditions were B: 15% (5 min) ,B: 30% (10 min) and B : 50% (30 min). The peak area and concentration of icariside Ⅰ ,epimedin C and icaiin respectively show good linear correlation r =0.999 3 ,r = 0.999 9 and r =0.999 7 in the range of 0. 20-1. 80 μg. The average recovery rates are 98.43% ,97.58% and 97.91% respectively. RSD (n = 3) is 2. 16% , 1. 77% and 1.90% separately. The method is simple, accurate and good reproducible for determining the mass fraction of icariside Ⅰ , epimedin C and icariin simultaneo usly.关键词
巫山淫羊藿/淫羊藿次苷Ⅰ/朝藿定C/淫羊藿苷/RP-HPLCKey words
Epimedium Wushanse/ icariside Ⅰ / epimedin C/ icariin/ HPLC分类
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周颖欣,张海荣,范代娣..不同产地不同部位巫山淫羊藿中黄酮的HPLC分析[J].化学工程,2013,41(2):6-8,3.
