激光生物学报2013，Vol.22Issue(1)：91-96,6.DOI:10.3969/j.issn.1007-7146.2013.01.015
秋水仙碱诱导亚麻四倍体的技术研究
A Technical Reseach on Induction of Flax Polyploid by Colchicine
摘要
Abstract
The mature seed of flax ShuangYa-7 as material, induction of flax polyploidy was conducted through flax seeds immersion of colchicines followed by sterile culture. Namely, the disinfected seeds were applied to induce the polyploidy after germination in perti dish, the polyploidy were induced by the treatment of colchicines. The results showed that the aberration rate of flax seeds is 40. 7% while the seeds after germination in water for 36 h were treated with 0. 075% concentration of colchicines for 24 h. Through observation of the external morphology, the stem of tetra-ploid is stronger than that of diploid, the leaf of tetraploid is thicker than that of diploid and the color of tetraploid is darker than that of diploid, the surface of tetraploid is rougher than that of diploid, the root of tetraploid is stronger than that of diploid and root number of branches are less than that of diploid.关键词
亚麻/秋水仙碱/诱导/多倍体Key words
Flax/ colchicines/ induction/ polyploid分类
农业科技引用本文复制引用
郭永霞,王丽艳,孙强,潘翠翠,殷奎德..秋水仙碱诱导亚麻四倍体的技术研究[J].激光生物学报,2013,22(1):91-96,6.基金项目
黑龙江省农垦总局科技计划重点攻关项目(HNK11A-01-07-08) （HNK11A-01-07-08）
黑龙江省自然科学基金(c201211) （c201211）
黑龙江省研究生创新科研项目(YJSCX2012-257HLJ) （YJSCX2012-257HLJ）
