热带作物学报2013，Vol.34Issue(1)：61-66,6.
剑麻愈伤组织的诱导和再生体系的建立
Establishment of SisalCallus Induction and Shoot Regeneration System
摘要
Abstract
The effects of explant types, combination and concentration of plant growth regulators on callus induction and shoot regeneration in Agave hybrid cv No.11648 were examined. The results showed that MS basal medium supplied with 6-BA and NAA was suitable for the light-yellow green callus induction, SH basal medium supplied with 6-BA, NAA and IBA was suitable for adventitious differentiation. The highest rate of light yellow-green, compact and nodular-like calli was formed on the MS medium supplemented with 3.0 mg/L BA in combination with 0.2 mg/L NAA, with 86.67% of shoot tips producing a compact, light yellow-green and nodular-.like callus. The calli derived from the different explants were subcultured on Schenk and Hildebrandt (SH) medium containing different concentrations of 6-BA, NAA and indole-3-butyric acid (IBA)for shoot induction. Shoot organogenesis was achieved when the callus tissue derived from shoot tips was transferred to the SH medium. Maximum organogenesis was obtained with 5.0 mg/L 6-BA in combination with 0.1 mg/L NAA and 0.1 mg/L IBA, yielding (98.33 ±1.67)% shoot induction frequencies and (13.19 ±0.58)adventitious differentiation coefficient. The regenerated shoots were rooted on hormone-free MS medium, with an average number of 5.39 roots per shoot, and a length of 8.44 cm per root within 30 days, and acclimatized in a greenhouse, with more than 95% survival. These results establish foundation for transgenic technology and germplasm innovation in sisal.关键词
剑麻/愈伤诱导/植株再生Key words
Sisal/Callus induction/Plant regeneration分类
农业科技引用本文复制引用
张燕梅,陈志,李俊峰,周文钊,陆军迎..剑麻愈伤组织的诱导和再生体系的建立[J].热带作物学报,2013,34(1):61-66,6.基金项目
中央级公益性科研院所基本业务费专项(No.sscri200802、No.1251022011010). （No.sscri200802、No.1251022011010）
