热带作物学报2013，Vol.34Issue(1)：67-73,7.
樟树优良家系的组培育苗技术研究
Tissue Culture and Micropropagation of Superior Families of Cinnamomum camphora(L.)Presl
摘要
Abstract
Two newly -selected superior families of Cinnamomum camphom were used for tissue culture and micropropagation. C. camphora explants displayed the optimum initial growth on the primary media, including improved basic DCR (DCR,)supplemented with 6-BA 0.3 mg/L and NAA 0.05 mg/L. Family X5 showed the highest shoot proliferation rate of 2.69 times on subculture DCR1+6-BA 0.6 mg/L+NAA 0.05 mg/L and family X9 exhibited the highest rate of 3.25 times on subculture DCR1+6-BA 1.0 mg/L+IAA 0.2 mg/L+NAA 0.05 mg/L. An optimization experiment revealed one common rooting medium: 1/2MS+IBA 2.0 mg/L+IAA 1.7 mg/L+6-BA 0.1 mg/L+NAA 0.05 mg/L, for two families. The rooting rate was 96.30%. Suitable techniques and procedures were identified for acclimatization, transplantation and later-period management of tissue-cultured plantlets. The survival rates of two families were 85.2% and 89.7%, respectively.关键词
樟树/优良家系/组织培养Key words
Cinnamomum camphora(L.)Presl./Superior families/Tissue culture分类
农业科技引用本文复制引用
周丽华,蔡燕灵,曾令海,蔡静如,张谦..樟树优良家系的组培育苗技术研究[J].热带作物学报,2013,34(1):67-73,7.基金项目
北京林业大学林木育种国家工程实验室开放课题项目(No.FOP2010-12) （No.FOP2010-12）
广东省林业科技创新专项(No.2008/09/10/11KJCX001-01) （No.2008/09/10/11KJCX001-01）
中央财政林业科技推广示范项目(No.[2011]GDTK-01) （No.[2011]GDTK-01）
林业公益性行业科研专项经费子项目(No.20110400103). （No.20110400103）
