山西农业大学学报（自然科学版）2012，Vol.32Issue(5)：412-414,3.
Myo5a特异性片段原核表达重组质粒的构建
Construction of Prokaryotic Expression Recombinant Plasmid of Specific Fragment Human Myo5a
游荷花 1沈敬华2
作者信息
- 1. 山西医科大学汾阳学院,山西汾阳032200
- 2. 内蒙古医学院,内蒙古呼和浩特010000
- 折叠
摘要
Abstract
To construct prokaryotic expression recombinant plasmid PGEX-4T-3/Myo5a of Myo5a gene, PCR was used to obtain 131bp coding region of Myo5a. The Myo5a gene was digested by restrictive enzyme Bam HI and Xho I. The gene was inserted into the prokaryotic GST fusion protein expression plasmid pGEX-4T-3, to form PGEX-4T-3/ Myo5a. The recombinant plasmid PGEX-4T-3/Myo5a was identified to be correct.关键词
Myo5a/原核表达/重组质粒Key words
Myo5a/ Prokaryotic expression/ Recombinant plasmid分类
医药卫生引用本文复制引用
游荷花,沈敬华..Myo5a特异性片段原核表达重组质粒的构建[J].山西农业大学学报（自然科学版）,2012,32(5):412-414,3.
