山西农业大学学报（自然科学版）2012，Vol.32Issue(5)：425-428,4.
芽孢杆菌LP-3抗菌物质的初分离及抑菌活性测定
Isolation of the Antifungal Substances from Endophytic Bacillus LP-3 and Determination on Its Antifungal Activity
摘要
Abstract
Endophytic Bacillus strain LP-3 could against many kinds of plant pathogens. To study the antifungal activity of the antifungal substance produced by the strain LP-3, the active constituents were extracted with five organic solvents. The antifungal activity of extracts was compared with other two fungicides to A. alternata, the toxicities of the extracts were determined on nine kinds of plant pathogenic fungi. The results showed that the methanol extracts showed a perfect inhibition against A. alternata with 68. 4%, and had stronger antifungal activity against filament growth and conidia germination of A. alternata with EC50 of 1. 18 mg · L-1 and 3. 61 mg · L-1 , respectively, which ranged from procymidone to iprodione. The extracts also had a broad antifungal spectrum, which could inhibited the filaments of nine kinds of pathogenic fungi with the EC50 of 1. 18 - 67. 93 mg · L-1.关键词
芽孢杆菌/抗菌物质/萃取/抑菌活性Key words
Bacillus sp. /Antifungal substances/ Extract/ Antifungal activity分类
农业科技引用本文复制引用
张宝俊,张家榕,韩巨才,刘慧平..芽孢杆菌LP-3抗菌物质的初分离及抑菌活性测定[J].山西农业大学学报（自然科学版）,2012,32(5):425-428,4.基金项目
山西省青年科技研究基金(2012021025-2) （2012021025-2）
山西农业大学科技创新基金(201221) （201221）
山西省留学归国人员基金(2011-52) （2011-52）
山西省科技攻关项目(20110311013-1) （20110311013-1）
