实用医学杂志2012，Vol.28Issue(24)：4038-4040,3.DOI:10.3969/j.issn.1006-5725.2012.24.003
人细胞因子IL-12p70与结核分枝杆菌特异性抗原Ag85A融合基因重组卡介苗的构建及鉴定
Construction and identification of recombinant BCG expressing fusion protein of human Interleukin-12p70 and immunodominant antigen Ag85A of Mycobacterium tuberculosis
摘要
Abstract
Objective To construct a recombinant BCG expressing fusion protein of human IL-12p70-Ag85A, to lay the foundation for production of new tuberculosis vaccine. Methods Construct recombinant plasmid of rpMV361-IL-12p70-Ag85A using plasmid pMV361 as the carrier. Then, the recombinant plasmid was imported BCG genome by electric transformation mode to construct recombinant BCG vaccine of rBCG-IL-12p70-Ag85A.The recombinant BCG was certified by antibiotic selection, PCR amplification and sequencing of recombinant BCG genome and westem-blotting. Results The recombinant BCG was certified to be constructed successfully expressing fusion protein of IL-12p70-Ag85A. Conclusion Recombinant rBCG-IL-12p70-Ag85A is constructed successfully, and can highly express the target protein.关键词
重组卡介苗/IL-12p70/Ag85AKey words
Recombinant BCG/IL-12p70/Ag85A引用本文复制引用
邓仪昊,何红云,张本斯..人细胞因子IL-12p70与结核分枝杆菌特异性抗原Ag85A融合基因重组卡介苗的构建及鉴定[J].实用医学杂志,2012,28(24):4038-4040,3.基金项目
大理学院博士科研启动基金(编号:KYBS201103) （编号:KYBS201103）
