实用医学杂志2012，Vol.28Issue(24)：4047-4049,3.DOI:10.3969/j.issn.1006-5725.2012.24.007
CLU基因干扰肾癌786-O细胞差异基因表达的研究
Interference in the expression of differential genes of human renal cancer cell line 786-O by CLU gene
摘要
Abstract
Objective To investigate the gene expression profiles of human renal cancer cell line 786-O before or after lentivirus-mediated clusterin silence by gene expression array. Methods 3 samples treated with CLU-RNAi-LV and 3 samples with negative control LV were labeled with Cy3 fluorescence as probes. The mixed probes were hybridized with gene chip. Differentially expressed genes screening were detected by microarray scanner. Results There were 1 731 differentially expressed genes in the 6 samples, among which 1 154 were up-regulation genes and 577 were down-regulation genes. GO analysis showed that the numbers of down-regulated genes in biological processes, cellular component and molecular function were significantly higher than those of up-regulated genes. The top 3 up-regulation enrichment pathways were cell adhesion molecules, allograft rejection and phagosome, whereas the top 3 down-regulation pathways were mTOR signaling pathway, MARK signaling and non-small cell lung cancer. Conclusion Differentially expressed genes screening before or after clusterin silence in 786-O showed that CLU gene plays an important role in the renal cancer's occurrence and development.关键词
癌,肾细胞/Clusterin/786-O/基因表达谱Key words
Carcinoma, renal cell/Clusterin/786-O/Gene expression array引用本文复制引用
石华,邓军洪,郑少斌,王铸,曹开源,周亮,万华..CLU基因干扰肾癌786-O细胞差异基因表达的研究[J].实用医学杂志,2012,28(24):4047-4049,3.基金项目
广东省科技计划项目(编号:2009B030801053) （编号:2009B030801053）
广州市科技计划项目(编号:2009Z1-E381-02) （编号:2009Z1-E381-02）
