实用医学杂志2012，Vol.28Issue(24)：4061-4064,4.DOI:10.3969/j.issn.1006-5725.2012.24.012
EGCG对雨蛙素诱导的小鼠急性胰腺炎的治疗作用
The effect of epigallocatechin-3-gallate EGCG on mice with acute pancreatitis
摘要
Abstract
Objective To study the effect of epigallocatechin-3-gallate (EGCG) on the pancreatic injury in mice with acute pancreatitis (AP). Methods Fifty-four balb/c mice were randomly divided into 9 subgroups; Control group, AP induction group and treatment group with EGCG. Each group was divided into 8h.、12h、24h subgroups after the induction of AP (n = 6 in each subgroup). In the AP induction group, cerulein (50 μg/kg body weight) in 0.9% NaCl was intraperitoneally injected into animals every one hour for 7 times. In the EGCG treatment group, EGCG (25 mg/kg) was injected in abdominally 1, 3, and 6 hours after the induction of pancreatitis .Six mice from each subgroup were killed for evaluation 8., 12、24 h after the pancreatitis induction. The levels of AMS、 IL-6、 TNF-α、 MDA in serum and NF-κB activity were determined. HE staining was performed to evaluate the morphologic changes in animal pancreats. Results The level of AMS、IL-6、 TNF-α、 MOA in serum, the pancreatic injury severity and NF-kB activity in pancreas in AP group were markedly higher than those in the control group(P < 0.05, respectively). In the EGCG treatment group, the levels of determination items decreased significantly than those in the AP group (P < 0.05). Conclusion EGCG could reduce pancreatic tissue injury in mice after AP treatment through inhibiting the NF-kB and oxidative stress pathways.关键词
EGCG/AP/NF-κB/TNF-α/IL-6/MDAKey words
EGCG/AP/TNF-α/NF-κB/IL-6/MDA引用本文复制引用
杨卫振,脱红芳,彭彦辉,张万星,暴雷,杨月卿..EGCG对雨蛙素诱导的小鼠急性胰腺炎的治疗作用[J].实用医学杂志,2012,28(24):4061-4064,4.基金项目
河北省自然基金课题(编号:C200801284) （编号:C200801284）
