新疆农业科学2013，Vol.50Issue(1)：8-14,7.DOI:10.6048/j.issn.1001-4330.2013.01.002
棉花抗低温生理指标和分子标记鉴定体系研究
Study on the Physiological Indices and Identifiable System of Molecule Mark about Cotton Cold Resistance
摘要
Abstract
[Objective]The main purpose of this research was to study the cotton physiological indices under cold stress and to determine specific molecule mark primers. [Method] Thirty kinds of cotton materials which have good agronomy characters and resist different cold temperatures were dealt with 3℃ cold stress during 24 hours. Seven physiological indices were tested before and after the cold stress; a series of gene sequences related to cold resistance were found from Genbank, and primers were designed by biological software Oligo6. 0, and the molecule marks of peculiar primer about resistant cold were selected. [Result] Three physiological indices related to cotton cold resistance were identified, two pairs of specific molecule marks related to cotton cold resistance were screened, and five cold resistant materials were selected. [ Conclusion ] A system of cotton cold resistant identification by using physiological indices that were combined with the molecule marks were set up.关键词
棉花/低温胁迫/生理生化指标/特异引物Key words
cotton/ cold stress/ physiological indices/ specific primer分类
农业科技引用本文复制引用
聂新辉,尤春源,张大伟,孔杰,雷江荣,吕军,冯国礼,杨永林,万英..棉花抗低温生理指标和分子标记鉴定体系研究[J].新疆农业科学,2013,50(1):8-14,7.基金项目
新疆生产建设兵团博士基金项目(2009JC02) （2009JC02）
石河子市科技攻关项目(2009NY05) （2009NY05）
