新疆农业科学2013，Vol.50Issue(1)：57-64,8.DOI:10.6048/j.issn.1001-4330.2013.01.010
库尔勒香梨萼片脱落与宿存相关基因的差异表达分析
Differential Expression Analysis of Calyx Falling off Korla Fragrant Pears and Their Persistent Related Genes
摘要
Abstract
[ Objective ] The expression of differential gene was studied in calyx falling - off Korla Fragrant Pears and their persistent related genes through the method of DDRT - PCR in order to seek, screen and define the genes and blooming. [Method] Korla Fragrant Pear flower (the second flower and the fourth flower) of the same inflorescence of three anthesis stages (early full bloom, full bloom, the end of bloom) in the same tree were taken as material. Improved DDRT - PCR method was used to investigate the differential display of genes relate to calyx leaving and persistent related genes of Korla Fragrant Pears. [Result] The result showed that the differential display exist in early full bloom stage, 42 different expressed cDNA fragments were obtained and only 7 double - primer bands were amplified. The alignments of nucleotide and amino acid sequences indicated that there were 2 fragments which have high homology that might be closely related to SPL and MYB, the two translation factors important to regulation on plant phytohormone and flowering. The homology was 78% and 86.83% , respectively. [Conclusion] This study had established the system of DDRT - PCR to obtain genes which relate to calyx of leaving and persistent. It laid on a foundation for cloning full length of relative gene,which also makes insurance to the further studying its physiological and biochemical characteristics and functions of thd gene expression product.关键词
库尔勒香梨/萼片/DDRT-PCR/转录因子Key words
Korla Fragrant Pear/ calyx/DDRT -PCR/ translation factor分类
农业科技引用本文复制引用
董芳园,张飞,王钰婷,牛建新..库尔勒香梨萼片脱落与宿存相关基因的差异表达分析[J].新疆农业科学,2013,50(1):57-64,8.基金项目
国际科技合作项目(2009YD32) （2009YD32）
国家科技基础性工作专项(2012FY110100-5-3) （2012FY110100-5-3）
