畜牧兽医学报2013，Vol.44Issue(2)：250-255,6.
鸡胚和雏鸡接种ALV-J相关急性纤维肉瘤浸出液的致病性比较
Comparison of the Pathogenicity of ALV-J Related Acute Fibrosarcoma Extract Inoculated on Embryos and Chicks
摘要
Abstract
This experiment was conducted to compare the pathogenicity of ALV-J related acute fibrosarcoma extract inoculated on embryos and chicks. Acute fibrosarcoma extract was inoculated on yolk sac of 5-day-old-embryos, chorioallantoic membrane of 11-day-old-embryos and intraper-itoneal inoculation of 1-day-old SPF chicks to compare pathogenicity of different inoculation methods. The results showed that the mortality of yolk sac inoculation was 14/30 at the age of 18-22 days, the incidence of visceral sarcoma was 8/14. The mortality of Chorioallantoic membrane inoculation was 17/30 at the age of 18-22 days, the incidence of visceral sarcoma was 6/17. As to the pathogenicity of the chicks, 13 hatched chicks from the chorioallantoic membrane inoculation group were all died, 11 of them developed visceral sarcoma. These results indicated that the pathogenicity of chorioallantoic membrane inoculation is higher than those inoculated on yolk sac of 5-day-old-embryos and those intraperitoneal inoculation of 1-day-old SPF chicks. Chorioallantoic membrane inoculation not only caused high incidence of visceral sarcoma, but also the sarcoma occurs earlier and faster, so it can be used as further acute fibrosarcoma model of artificial disease.关键词
鸡胚接种/雏鸡/ALV-J(J亚群禽白血病病毒)/急性纤维肉瘤Key words
embryo inoculation/ chick/ different inoculation methods/ ALV-J (subgroup J avian leukosis virus)/ acute fibrosarcoma分类
农业科技引用本文复制引用
李德庆,赵鹏,王鑫,汪晓飞,崔治中..鸡胚和雏鸡接种ALV-J相关急性纤维肉瘤浸出液的致病性比较[J].畜牧兽医学报,2013,44(2):250-255,6.基金项目
国家自然科学基金面上项目(#31172330) （#31172330）
国家公益性行业(农业)科研专项经费项目(#20123055) （农业）
