畜牧兽医学报2013，Vol.44Issue(2)：322-328,7.
枸杞多糖对雏鸡淋巴细胞体外增殖及分泌IL-2的影响
Effects of Lycium barbarum Polysaccharide on Cell Proliferation of Chicken Lymphocytes and IL-2 Excretion in vitro
摘要
Abstract
This experiment was conducted to investigate the effects of Lycium barbarum polysaccharide (LBP) on cell proliferation of chicken Lymphocytes in vitro. Different concentrations of LBP were added into cultured with plants hemagg-lutinin (PHA) stimulated chicken thymus, spleen and peripheral blood lymphocyte, and cultured with bacteria lipopolysaccharide (LPS) stimulated chicken bursa of Fabricius, peripheral blood lymphocyte, spleen, MTT method and ELISA method were used to determine the lymphocyte proliferation and IL-2 excretion respectively. The results showed that: (1) LBP could stimulate T, B lymphocyte proliferation and IL-2 excretion in certain concentration range and with the increase of the concentration of polysaccharide increase; (2) The OD value of T, B lymphocyte with the source of the same organ were significantly growth trend with chickens dayage growth in same concentration of LBP. (3) The OD value of thymus T lymphocyte and bursa of Fabricius B lymphocyte were higher than in peripheral blood and the spleen T, B lymphocytes with the same concentration LBP and the same day age of chicken. These results indicated that LBP could stimulate T, B lymphocyte proliferation and IL-2 excretion, and the promoting function of lymphocyte proliferation have certain concentration-response, time relationship and organizational correlation.关键词
雏鸡/枸杞多糖/淋巴细胞增殖/IL-2Key words
chicken/ LBP/ lymphocyte proliferation/IL-2分类
农业科技引用本文复制引用
徐占云,秦睿玲,褚耀诚,李国辉,李春红,王丽..枸杞多糖对雏鸡淋巴细胞体外增殖及分泌IL-2的影响[J].畜牧兽医学报,2013,44(2):322-328,7.基金项目
河北北方学院资助课题(2010016) （2010016）
