应用生态学报2012，Vol.23Issue(7)：1913-1920,8.
缘管浒苔和浒苔对海水盐度胁迫的生理响应
Physiological responses of Enteromorpha linza and Enteromorpha prolifera to seawater salinity stress
摘要
Abstract
To investigate the physiological responses and adaptation mechanisms of Enteromorpha to seawater salinity stress, a laboratory experiment with Enteromorpha linza and E. prolifera was conducted to study their fresh mass (FM) , relative growth rate (RGR) , relative electrical conductivity ( REC) , chlorophyll ( Chl) and carotenoid ( Car) contents, Chl a/Chl b, Chl/Car, chlorophyll fluorescence parameters, and osmotic adjustment ability (OAA) under the stress of different salinity levels of diluted and concentrated seawater for 10 days. Compared with the control, 10%-200% salinity seawater increased the FM and RGR of the two Enteromorpha species obviously, 100% and 50% salinity seawater made the FM and RGR of E. linza and E. prolifera peaked, respectively, while 300% salinity seawater decreased the FM and RGR of E. linza and E. prolifera significantly,with the decrement being larger for E. linza. The biomass of E. linza and E. prolifer only had an increase in 50% and 100% sanity seawater and in 10% , 50% , 100% , and 200% salinity seawa-ter, respectively. The Chl and Car contents and Chl a/Chl b of E. linza and E. prolifera had a significant increase in 10% salinity seawater, but decreased after an initial increase with the increasing salinity level of seawater. The Chl and Car contents and Chl a/Chl b of E. linza and E. prolifera peaked in 100% and 50% salinity seawater, respectively. With increasing salinity of seawater, the light use efficiency ( α) , maximal photochemical efficiency of PSⅡ( Fv/Fm ) , actual photochemical efficiency of PS II in the light ( Yield) , maximal relative electron transport rate ( rETRmax ) , and half-saturation light intensity (Ik) of E. linza and E. prolifera all showed the same variation trend as Chl. 10% -300% salinity seawater enabled E. linza and E. prolifera to express certain osmotic adjustment ability (OAA) , and the OAA of E. linza and E. prolifer peaked in 100% and 50% salinity seawater, respectively. The growth of Enteromorpha had no correlation with Chl/Car, but was significantly negatively correlated with REC and positively correlated with Chl, Car, Chl a/ Chl b, Fv/Fm, Yield, rETRmax α, Ik,and OAA. To sum up, 100% salinity was the optimal salt concentration for the growth of E. linza, and 50% salinity was optimal for E. prolifera. E. prolifera could adapt to a wider range of salinity than E. linza. The parameters REC, Chl, Car, Chl a/Chl b, Fv/Fm, Yield, rETRmax, α, Ik, and OAA could be used to evaluate the salt adaptation of Enteromorpha.关键词
缘管浒苔/浒苔/海水/生长/叶绿素荧光参数/渗透调节能力Key words
Enteromorpha linza/ Enteromorpha prolifera/ seawater/ growth/ chlorophyll fluorescence parameter/ osmotic adjustment ability.分类
生物科学引用本文复制引用
高兵兵,郑春芳,徐军田,郑青松,刘兆普,翟瑞婷,蒋和平..缘管浒苔和浒苔对海水盐度胁迫的生理响应[J].应用生态学报,2012,23(7):1913-1920,8.基金项目
国家科技支撑计划项目(2008BAD95B05)和浙江省近岸水域生物资源开发与保护重点实验室开放项目(2010F30003)资助. （2008BAD95B05）
