应用生态学报2012，Vol.23Issue(7)：1952-1958,7.
大豆胰蛋白酶抑制剂和防御信号物质对斜纹夜蛾解毒酶的影响
Effects of soybean trypsinase inhibitor and defense signaling compounds on detoxification enzymes in Spodoptera litura (F.) larvae
摘要
Abstract
In a long history of interactions between insects and plants, plants have developed various anti-insect compounds and defense signaling transduction pathways to defend against herbivorous insects, while insects have responded with sophisticated detoxification enzyme systems to protect against the toxicity of anti-insect compounds. In this study, the 2nd or 3rd instar of Spodoptera litura larvae were successively fed with the diets containing 0. 5% soybean trypsinase inhibitor ( SBTI) for six generations to evaluate the effects of SBTI and defense signaling compounds on the activities of detoxification enzymes carboxylesterase (CarE) and glutathione-S-transferase (GST) in the mid-gut and fatbody of the larvae. After fed with the diets, the CarE and GST activities in the 5th instar larvae increased significantly. The CarE activity in the midgut and fatbody of the second generation larvae was the highest, being 2.06 and 2.40 times, and 1. 96 and 2.70 times of that of the control, and the GST activity in the midgut and fatbody of the fourth and second generations was the highest, being 7.03 and 11.58 times, and 5.71 and 3.60 times of that of the control, respectively. These induced enzyme activities decreased gradually when the larvae continuously grew with the SBTI-containing diets. In addition, when the S. litura larvae were pre-exposed to methyl jasmonate (MeJA) or methyl salicylate (MeSA) for 48 h or fed with the diets containing 0. 5% SBTI, the activities of CarE and GST in the midgut and fatbody increased significantly, and, when the 2nd instar larvae were pre-exposed to MeJA and MeSA for 48 h, the effects of SBTI on the GST activity in larval midgut and fatbody were reduced.关键词
蛋白酶抑制剂/谷胱甘肽转移酶/羧酸酯酶/茉莉酸甲酯/水杨酸甲酯Key words
protease inhibitor/ glutathione-S-transferase/ carboxylesterase/ salicylates/ jasmonates分类
农业科技引用本文复制引用
吴国昭,胡林,叶茂,王瑞龙,朱克岩,曾任森,蔡文..大豆胰蛋白酶抑制剂和防御信号物质对斜纹夜蛾解毒酶的影响[J].应用生态学报,2012,23(7):1952-1958,7.基金项目
国家自然科学基金海外学者合作研究项目(31028018)、广东省自然科学基金项目(S2011040004336)和广东省高等学校珠江学者岗位计划项目(2010)资助. （31028018）
