应用生态学报2012，Vol.23Issue(10)：2923-2930,8.
土壤宏蛋白质组学在土壤污染评价中的应用
Applications of soft metaproteomics in soil pollution assessment: A review
摘要
Abstract
Soil microbial indicator is one of the important biological indicators in evaluating the extent of soil contamination. In recent years, with the development of molecular biology, many studies have focused on the ecological functions of soil microorganisms by using metagenomics, metatran-scriptome and metaproteomics. Relative to metagenomics and metatranscriptome, soil metaproteomics aims to investigate the spatial and temporal changes of the proteins extracted from soil as well as the functional components of soil microbial genomic expression products, which is more conclusive to explore the ecological functions of soil microbes and their roles in soil pollutants transportation and transformation. Therefore, soil metaproteomics has great potential in soil pollution assessment. Currently, soil metaproteomics is still at its infancy stage, while soil protein extraction method is one of the key factors restraining the potential application of soil metaproteomics. In this paper, the advantages and disadvantage of soil metaproteomics in soil pollution assessment were reviewed , with the focus on the comparison of different soil protein extraction methods. In combining with case studies, the feasibility and limits of soil proteins as an indicator for soil pollution assessment were analyzed. In addition, the future research perspectives on the development of soil metaproteomics were discussed.关键词
土壤污染/土壤微生物/宏蛋白质组学/土壤蛋白质提取方法Key words
soil pollution/ soil microbe/ metaproteomics/ soil protein extraction method分类
资源环境引用本文复制引用
张曦,李锋,刘婷婷,陈英旭..土壤宏蛋白质组学在土壤污染评价中的应用[J].应用生态学报,2012,23(10):2923-2930,8.基金项目
国家自然科学青年基金项目(21007055)和中国博士后科学基金项目(20100471714,201104718)资助. （21007055）
