应用生态学报2012，Vol.23Issue(11)：3016-3020,5.
高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响
Effects of high temperature and humidity on leaf Bt protein expression of transgenic Bt cotton
摘要
Abstract
Different origins Bt cotton cultivars, including DP410B (conventional cultivar) and Dai-za No. 1 (hybridized cultivar) from US and Sikang No. 1 (conventional cultivar) and Sikang No.3 (hybridized cultivar) from China, were taken as the test materials to investigate the effects of high temperature (37 ℃) and different humidity (50% , 70% , and 90% ) on the leaf Bt protein expression of Bt cotton. At high temperature, temperature and humidity had no significant effects on the leaf Bt protein expression of the cultivars at peak squaring stage. At peak flowering stage, as compared with the control (25-30 ℃ and 60% -70% humidity) , 37 ℃ and 50% humidity decreased the leaf Bt protein content of conventional cultivars significantly by 2. 6% -3.0% . At peak boiling stage, compared with the control, 37℃ and 50% humidity decreased the leaf Bt protein content of DP410B, Sikang No. 1, and Sikang No. 3 significantly by 3. 3% -5. 8% . Among the four cultivars, DP410B and Daiza No. 1 had the highest leaf Bt protein content, while Sikang No. 1 had the lowest one.关键词
Bt棉/Bt蛋白/高温/湿度Key words
Bt cotton/ Bt protein/ high temperature/ humidity分类
农业科技引用本文复制引用
张祥,王桂霞,顾超,韩勇,许映飞,陈源,陈德华..高温和湿度对转Bt基因棉叶片Bt蛋白表达的影响[J].应用生态学报,2012,23(11):3016-3020,5.基金项目
国家自然科学基金项目(30971727,31171479)、国家农业现代产业技术体系岗位专家项目(CARS-18-20)、江苏省高校优势学科建设工程项目、江苏省重点实验室重大项目(10KJA210057)和高等学校博士学科点专项科研基金项目(20113250110001)资助. （30971727,31171479）
