中国康复2012，Vol.27Issue(6)：403-405,3.DOI:10.3870/zgkf.2012.06.001
重复经颅磁刺激对脑梗死后海马PSD-95和GAP-43表达的影响及其机制研究
Effects and mechanism of repetitive transcranial magnetic stimulation (rTMS) on expression of PSD-95 and GAP-43 in hippocampus of rats with cerebral infarction
摘要
Abstract
Objective:To investigate the effects of high-frequency repetitive transcranial magnetic stimulation (rTMS) on the expression of PSD-95 and GAP-43 and its mechanism in the ischemic hippocampus of rats with cerebral infarction. Methods: A total of 20 SD rats were divided into model control group( A) ,rTMS group(B) ,with 6 cases in every group,rTMS+NS group(C) and rTMS+H89 group(D), with 4 cases in every group. Reperfusion model with middle cerebral artery occlusion was established. rTMS of 20Hz was given to successful models for 7 days. Utrastructures of the ischemic CA1 and expression changes of PSD-95 and GAP-43 in rats of group A and B were investigated under the transmission electron microscopy and western blot respectively. The expression of pCREB, PSD-95 and GAP-43 were detected in the group C and D. Results: As compared with group A, the PSD-95 and GAP-43 expression levels were increased in group B(P<0. 01). Under the transmission electron microscopy,the synaptic volume was increased,the anterior and posterior electron dense areas of the synapse were widened,the electron density and synaptic vesicles quantity were increased in group B. As compared with group C, the expression levels of pCREB,PSD-95and GAP-43 were decreased in group D(P<0. 01). Conclusion: rTMS could promote PSD-95 and GAP-43 expression in the ischemic hippocampus after cerebral ischemia probably by regulating the PKA-CREB pathway.关键词
脑梗死/重复经颅磁刺激/PKA-CREB/PSD-95/GAP-43Key words
cerebral infarction/rTMS/PKA-CREB/PSD-95/GAP-43分类
医药卫生引用本文复制引用
赵秀秀,韩肖华,郭风,于利国,向艳平,黄晓琳..重复经颅磁刺激对脑梗死后海马PSD-95和GAP-43表达的影响及其机制研究[J].中国康复,2012,27(6):403-405,3.基金项目
教育部高等学校博士学科点专项科研基金(20090142110013),国家自然科学基金(81071601),国家自然科学基金(81171858) （20090142110013）
