中国临床药理学杂志2013，Vol.29Issue(2)：139-141,3.
α-细辛醚对癫痫大鼠的海马钙稳态调控的影响
Effects of α-asarone on modulation of hippocampus calcium homeostasis in epilepsy rats
摘要
Abstract
Objective To observe the effects of α- asarone on hippocampus calcium homeostasis in in epilepsy rats. Methods Rat status epilepticus (SE ) model was induced by Lithium - Pilocarpine. The effects of α - asarone on the content of intracellular free Ca2 + , the average passage fluorescence of relative activity of calmodulin ( CaM ) and the expression of the CaM and Ca2 + /CaM - dependent kinase Ⅱa ( CamK Ⅱa) in hippocampus of post - SE rats were observed. Results The intracellular free Ca2 + content was significantly increased in the hippocampus of epilepsy rats (P < 0. 05 ). The mean channel fluorescence of intracellular free CaM was significantly decreased at different time post - SE (P < 0. 05). At 12 - 72 h post - SE, the CaM expression in model group was significantly higher than those in normal group, and still higher than in normal group at one week post - SE. The expression of CaMK Ⅱa -mRNA was significantly decreased at different time in the hippocampus of post - SE rats ( P < 0. 05 ) , and still lower than in normal group at one week post - SE. The content of intracellular free Ca + and the expression of CaM in α - asarone group were significantly lower than those in model group at different time in the hippocampus of post - SE rats (P < 0. 05 ). The mean channel fluorescence of intracellular free CaM and the expression of CaMK Ⅱa - mRNA in α - asarone group were significantly higher than those in model group (P < 0. 05 ) . Conclusion α - asarone could significantly downregulated the intracellular free Ca + content and the expression of CaM and upregulated the expression of CamK Ⅱa in the hippocampus of post - SE.关键词
癫痫/α-细辛醚/钙调蛋白Key words
epilepticus/ α - asarone/ calmodulin分类
医药卫生引用本文复制引用
苗静琨,陈启雄,李春,李晓文,刘振球,吴小玫,张晓萍..α-细辛醚对癫痫大鼠的海马钙稳态调控的影响[J].中国临床药理学杂志,2013,29(2):139-141,3.基金项目
重庆医科大学博士启动基金资助项目(0200101060) （0200101060）
