中国农业大学学报2013，Vol.18Issue(2)：86-92,7.
黄瓜种传镰刀菌种类的鉴定及其致病性研究
Study on identification of cucumber seedborne Fusarium species and testing of their pathogenicity
摘要
Abstract
In order to study the seedborne Fusarium species and their harmness of cucumber seeds of popularly planted varieties,cucumber seeds of twenty-one varieties from cucumber-growing regions in China were collected for Fusarium detection. Nine Fusarium isolates were isolated from seed embryos and seed surface. The isolates were identified using morphology and DNA sequencing analysis. Effects on seed germination and pathogenicity of these Fusarium isolates were determined.Results showed that the nine Fusarium isolates belong to three species,i.e. Fusarium oxysporum (4 isolates),F. moniliforme (4 isolates) and F. prolifertum (1 isolate). The four F. oxysporum isolates had significant effect on seed germination,compared to the control treatment. The values of the germination index, the seedling vigor index,root length and fresh weight of seedlings decreased significantly in the treatment of F. oxysporum. Results also showed that the isolates of F. oxysporum were pathogenic to seedlings of cucumber and caused typical wilt symptoms on leaves,fulfilling Koch's postulates. All the other five isolates of Fusarium had suppressive effect on seed germination of cucumber, but they were not pathogenic to cucumber seedlings. To our knowledge, this is the first report of the seedborne F. oxysporum pathogenic to cucumber seedlings in China.关键词
黄瓜/种传镰刀菌/种类鉴定/种子发芽/致病性Key words
cucumber/ seedborne Fusarium oxysporum /species identification/ seed germination/ pathogenicity分类
农业科技引用本文复制引用
蒋荷,李旭,郑慧慧,吴学宏,杨腊英..黄瓜种传镰刀菌种类的鉴定及其致病性研究[J].中国农业大学学报,2013,18(2):86-92,7.基金项目
公益性行业(农业)科研专项经费项目资助(200903049) （农业）
