中国人兽共患病学报2013，Vol.29Issue(3)：232-236,5.DOI:10.3969/cjz.j.issn.1002-2694.2013.03.005
山东省肠道病毒71型分离株SDLY11全基因组序列分析
Complete genome sequence analysis of enterovirus 71 SDLY 11 strain isolated in Shandong, China
摘要
Abstract
The present study aimed to investigate the complete genome sequence of enterovirus 71 (EV71) SDLY 11 strain isolated in Linyi, Shandong Province in 2009 and analyze the candidate sites that were related to neurovirulence . EV71 SDLY 11 strain was isolated from the patients ' feces sample. The whole viral genome was obtained by RT-PCR and the sequence was analyzed by DNAStar and MEGA 4. The genome of SDLY 11 had 7 405 nucleotides , which contained 5 UTR (742 nt), 3'UTR(84 nt) and ORF (6 579 nt). A polyprotein with 2 193 amino acids was encoded by ORF . Phylogenetic analysis of VP1 and complete sequences revealed that SDLY 11 was located in the branch of C4 subtype. Homology analysis revealed that SDLY 11 had the most similarity with Fuyang strain . Two mutations (T40C , C575T) in 5 UTR and one mutation (T144S) in VP2 were found in strains from HFMD patients with CNS by nucleotide sequence analysis . SDLY 11 belongs to C4 sub-genotype. These results suggested that nucleotide mutations T40C and C575T in 5'UTR and T144S in VP2 may be relevant to the neurovirulence of EV71 .关键词
肠道病毒71型/手足口病/系统进化分析/同源性分析/神经毒力Key words
enterovirus 71/ hand, foot and mouth disease / phylogenetic analysis / homology analysis / neurovirulence分类
医药卫生引用本文复制引用
袁晓晶,温红玲,高峰,许洪芝,司鲁莹,王东旭,赵丽,王志玉..山东省肠道病毒71型分离株SDLY11全基因组序列分析[J].中国人兽共患病学报,2013,29(3):232-236,5.基金项目
国家自然科学基金(30901265) （30901265）
山东省自然科学基金(ZR2010CQ030) （ZR2010CQ030）
教育部博士点基金新教师基金(20090131120068) （20090131120068）
山东大学公共卫生学院青年人才创新基金(GGWS200801)资助 （GGWS200801）
