中国人兽共患病学报2013，Vol.29Issue(3)：278-281,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.03.014
PCR方法检测猕猴奇异变形杆菌
Detection of Proteus mirabilis in Macaca mulatta by PCR method
摘要
Abstract
In order to provide data for Proteus mirabilis identification , clinical diagnosis , treatment and epidemiological investigation in Macaca mulatto, the Proteus mirabilis from Macaca mulatto was detected by PCR method in this study . Proteus mirabilis was isolated and identified from feces of Macaca mulatta, and the DNA was extracted from cultured strains and then amplified by PCR . The products were analyzed by gel electrophoresis and then sequencing . The sequence of Proteus mirabilis was identified by BLAST . The specificity and sensitivity of PCR were assessed . Eighty samples of Macaca mulatta were screened for Proteus mirabilis by PCR method and traditional method of isolation and identification simultaneously . Results showed that the sequence (225 bp) of Proteus mirabilis was amplified from Macaca mulatta and submitted to GenBank (No . JQ040548); homology analysis showed that the strain shared 98 .2% similarity with the Proteus mirabilis (AM942759). The specificity was identified by negative amplification of other 5 non-Proteus mirabilis strains. The sensitivity of PCR was 80 cfu/mL . The PCR method was comparable with traditional method of isolation and identification as the positive rate of Proteus mirabilis in Macacamulatta detected by that was 2 .5% (2/80) . The PCR method was useful for its rapid and accurate detection of Proteus mirabilis in Macacamulatta, which would be applicable in clinic.关键词
猕猴/奇异变形杆菌/PCR检测Key words
Macacamulatta/ Proteus mirabilis / PCR detection分类
医药卫生引用本文复制引用
禹文海,赵远,黄芬,杨凤梅,鲁帅尧,李艳艳,陈丽雄,王俊斌,和占龙..PCR方法检测猕猴奇异变形杆菌[J].中国人兽共患病学报,2013,29(3):278-281,4.基金项目
云南省应用基础研究面上项目(2009ZC184M) （2009ZC184M）
云南省技术创新人才培养基金(2009CI117).(禹文海和赵远在本项工作中具有同等贡献) （2009CI117）
