中国兽医科学2013，Vol.43Issue(2)：131-135,5.
麇鹿朊蛋白全基因的克隆及序列分析
Cloning and sequencing of PRNP gene from Milu deer in China
摘要
Abstract
To get prion protein(PRNP) complete encoding gene of Milu deer,and to analyse its homol-ogy by comparing sequences from different animals, a pair of specific primers were designed based on the sequence of PRNP gene of Cervus elaphus in GenBank. The complete PRNP gene of Milu deer was amplified from total genomic DNA by PCR and was cloned into pGEM-T. The cloning product was sequenced and then analyzed by comparing the sequence with those from the other animals using the software of DNAS-tar. The result indicated that the complete PRNP gene of Milu deer was successfully amplified. The recom-binant plasmid pGEM-T-ML with PRNP gene of Milu deer was constructed. By sequence analysis,ORF of the cloned PRNP gene was 771 bp in length, which encodes a protein of 256 amino acids with molecular weight of about 28 200 u. The similarity of both nucleotide acid and amino acid sequences is more than 98. 8% when compared with the published sequences in GenBank,and it was from 96. 5% to 98. 4% when compared with GenBank published sequences of non-cervidae. In conclusion,there was strong conservation of nucleotide acid and amino acid of sequences of PRNP between Milu deer and the other cervidae species. High similarity between Milu deer and several non-cervidae indicated that PRNP is a kind of ancient protein with strong conservation.关键词
麋鹿/朊蛋白全基因/克隆/序列分析/鹿慢性消耗性疾病Key words
Milu deenprion protein(PRNP) gene/cloning/sequence analysis/cervid chronic wasting disease分类
农业科技引用本文复制引用
刘雨田,孙成友,李娟,迟田英,张鑫鑫,于小静,王志亮..麇鹿朊蛋白全基因的克隆及序列分析[J].中国兽医科学,2013,43(2):131-135,5.基金项目
国家公益性行业(农业)科研专项(200903037) （农业）
