中国兽医科学2013，Vol.43Issue(2)：146-151,6.
牦牛源多杀性巴氏杆菌外膜蛋白OmpH和OmpA重组亚单位疫苗对小鼠的免疫效果
Immune efficacy of recombinant subunit vaccine with outer membrane proteins H and A against yak Pasteurella multocida in mice model
摘要
Abstract
In order to study the protection of OmpH and OmpA proteins of Pasteurellosis multocida derived from yak, the prokaryotic expression plasmid pET28a-OmpH and pET28a-OmpA were constructed. The vaccines were prepared with the purified recombinant outer member protein H(rOmpH) and the recombinant outer member protein A(rOmpA). The mice were hypodermicly immunized with rOmpH, rOmpA,rOmpH-A vaccines and the inactivated whole bacteria vaccine, respectively and PBS was used as negative control. Sera were collected each week from the mice to examine antibodies after first immunization using an indirect ELISA. The immunized mice were challenged with 2LD50 yak P. multocida on day 9 after third immunization. In result,the protection rate of the inactivated whole bacteria vaccine and rOmpA were 80% and 40% respectively, but mice in the other groups were dead. The rOmpH and rOmpH-A groups only postponed the time of mouse death, and the protectivity was weak and the immunization was unable to resist the lethal attack using homologous strain. The results showed that the rOmpA protein had protective efficacy,and the rOmpH had not protective efficacy.关键词
牦牛/多杀性巴氏杆菌/ompA基因/ompH基因/重组蛋白Key words
yak/Pasteurella multocida /0mpH gene/OmpA gene/recombinant protein分类
农业科技引用本文复制引用
田亮,康立超,赵文娟,薄新文,马勋..牦牛源多杀性巴氏杆菌外膜蛋白OmpH和OmpA重组亚单位疫苗对小鼠的免疫效果[J].中国兽医科学,2013,43(2):146-151,6.基金项目
新疆农垦科学院科技引导计划项目(YYD201107) （YYD201107）
新疆农垦科学院青年基金项目(YQJ2009-02,YQJ201110) （YQJ2009-02,YQJ201110）
