猪巨细胞病毒gB基因优势抗原表位区的原核表达及间接ELISA方法的建立OA北大核心CSCDCSTPCD
Prokaryotic expression of immunodominant region of PCMV gB gene and development of an indirect ELISA for detecting antibodies against porcine cytomegalovirus
扩增了猪巨细胞病毒(PCMV) gB基因的优势抗原表位区,将构建成功的gB基因优势抗原表位区原核表达重组质粒(pET32a-gB)转化入表达菌Rosetta(DE3)中进行表达,以表达的重组蛋白作为包被抗原建立了一种检测PCMV抗体的间接ELISA方法,并对该间接ELISA检测方法进行了优化.结果显示,最佳抗原包被浓度为1.9 μg/mL,最佳血清稀释度为1∶160,二抗最佳稀释度为1∶10 000；当样品D450nm≥0.26时判为阳性,当样品…查看全部>>
In this study,the major epitope region of porcine cytomegalovirus(PCMV) gB gene was amplified. The prokaryotic expression recombinant plasmid with the gB major epitope region(pET32a-gB) was constructed and expressed with Rosetta(DE3) prokaryotic expression system. The purified recombinant protein was used as coating antigen in the development of an indirect ELISA method for detecting antibodies against PCMV. The ELISA detection method was optimized with the …查看全部>>
刘骁;廖珊;朱玲;周远成;周璐
四川农业大学动物生物技术中心,四川雅安625014四川农业大学动物生物技术中心,四川雅安625014四川农业大学动物生物技术中心,四川雅安625014动物疫病与人类健康四川省重点实验室,四川雅安 625014四川农业大学动物生物技术中心,四川雅安625014
农业科技
猪巨细胞病毒原核表达gB基因优势抗原表位区蛋白间接ELISA
porcine cytomegalovirusprokaryotic expressiongB major epitope regionindirect ELISA
《中国兽医科学》 2013 (2)
152-158,7
四川省青年科技基金项目(200930421)
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