中国兽医科学2013，Vol.43Issue(2)：177-183,7.
牦牛S100A12基因的克隆及其在生殖器官中表达情况的检测
Cloning of Bos grunniens S100A12 gene and detection of its expression profile in tissues of the reproductive system
摘要
Abstract
The purpose of the present research was to investigate expression of S100A12 gene in the reproductive system of yak(Bos grunniens). S100A12 cDNA derived from spleen was cloned using RT-PCR, then expression of S100A12 gene and protein were detected by semi-quantitative RT-PCR and immunohis-tochemical staining from spleen,lymph nodes,mammary gland and reproductive system. The result showed that the yak S100A12 cDNA fragment is 279 bp in size, which contains a complete open reading frame (ORF) and encodes 92 amino acids. The relative expression of S100A12 mRNA and protein was the highest in spleen,moderate in testis,epididymis,lymph nodes,mammary gland and ovary,and the lowest in penis, vagina,uterine(horn,body and cervix). The results indicated that S100A12 mRNA and protein were expression in all tissues of the reproductive system, which suggested that S100A12 gene may play a role in reproduction of yak.关键词
牦牛/S100A12基因/组织表达Key words
yak(Bos grunniens) /S100A12 gene/tissue expression分类
农业科技引用本文复制引用
曹随忠,崔亚飞,姚学萍,雍康,杨德英,沈留红,余树民,刘宗平..牦牛S100A12基因的克隆及其在生殖器官中表达情况的检测[J].中国兽医科学,2013,43(2):177-183,7.基金项目
中国博士后科学基金项目(20090451250) （20090451250）
四川省教育厅青年基金项目(09zb054) （09zb054）
教育部长江学者和创新团队发展计划创新团队项目(IRT0848) （IRT0848）
科技部国际科技合作重点项目(2005DFA30720) （2005DFA30720）
