首页|期刊导航|中华医学杂志（英文版）|Exposure to heat-inactivated Trichophyton rubrum resulting in a limited immune response of human keratinocytes
中华医学杂志（英文版）2013，Vol.126Issue(2)：215-219,5.DOI:10.3760/cma.j.issn.0366-6999.20122562
Exposure to heat-inactivated Trichophyton rubrum resulting in a limited immune response of human keratinocytes
Exposure to heat-inactivated Trichophyton rubrum resulting in a limited immune response of human keratinocytes
摘要
Abstract
Background Trichophyton rubrum (T.rubrum) represents the most important agent of dermatophytosis in humans.T.rubrum infection causes slight inflammation,and tends to be chronic and recurrent.It is suggested that it may result from the failure of epithelial cells to recognize T.rubrum effectively and initiate effective immune responses.The C-type lectin receptors (CLR) and toll-like receptors (TLR) are the two major pattern recognition receptors (PRRs) that recognize fungal components.Therefore,the purpose of the study was to analyze the expression of those PRRs and the cytokines in HaCaT cells stimulated with heat-inactivated T.rubrum conidia and hyphae,respectively.Methods HaCaT cells were unstimulated or stimulated with heat-inactivated T.rubrum conidia and hyphae (1×106 and 1.5×10s colony-forming unit (CFU) in 2 ml medium,respectively) for 6,12 and 24 hours.The mRNA expression of PRRs involved in recognizing fungal pathogen-associated molecular patterns (PAMPs) and signaling molecules were measured by quantitative reverse transcription polymerase chain reaction (RT-PCR).Meanwhile,surface toll-like receptor (TLR) 2,TLR4 and Dectin-1 were analyzed by fluorescence-activated cell sorter (FACS) 24 hours after treatment.The cytokines were detected in cell culture supernatants of HaCaT cells in 12 and 24 hours after treatment.Results HaCaT cells constitutively expressed mRNA of membrane-bound TLR1,2,4 and 6,Dectin1 and DC-SIGN,but not Dectin-2 or Mincle.Heat-killed T.rubrum did not significantly upregulate gene transcriptions of the PRRs of HaCaT cells.Heat-inactivated T.rubrum conidia significantly reduced the surface expression of TLR2 and Dectin-1,and suppressed the secretions of interferon-inducible protein-10 (IP-10) and monocyte chemotactic protein-1 (MCP-1) of HaCaT cells,while heat-killed T.rubrum hyphae significantly induced the secretions of IP-10 and MCP-1.Conclusion The cell-wall antigens of T.rubrum fail to activate transcriptional expression of PRRs and induce a lower immune response of HaCaT cells by limited cytokines secretion.关键词
human keratinocytes/ Trichophyton rubrum/ pattern recognition receptors/ immune responseKey words
human keratinocytes/ Trichophyton rubrum/ pattern recognition receptors/ immune response引用本文复制引用
HUANG Xiao-qiang,YI Jin-ling,YIN Song-chao,CHEN Rong-zhang,LI Mei-rong,GONG Zi-jian,LAI Wei,CHEN Jian..Exposure to heat-inactivated Trichophyton rubrum resulting in a limited immune response of human keratinocytes[J].中华医学杂志（英文版）,2013,126(2):215-219,5.基金项目
This work was supported by the grants from the Fundamental Research Funds for the Central Universities (No.10ykpy04) and the National Natural Science Foundation of China (No.30600028). （No.10ykpy04）
