中华医学杂志（英文版）2013，Vol.126Issue(3)：471-475,5.DOI:10.3760/cma.j.issn.0366-6999.20112546
Radiolabeling LyP-1 peptide and preliminary biodistribution evaluation in mice bearing MDA-MB-435 xenografts
Radiolabeling LyP-1 peptide and preliminary biodistribution evaluation in mice bearing MDA-MB-435 xenografts
YU Ming-ming 1WANG Rong-fu 2CHEN Yue-hua 1ZHOU Hai-zhong 1DENG Xiao-hu1
作者信息
- 1. Department of Nuclear Medicine, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, China
- 2. Department of Nuclear Medicine, Peking University First Hospital,Beijing 100034, China
- 折叠
摘要
Abstract
Background Recent studies have shown the LyP-1 peptide can home to either tumor lymphatics or the tumor cells and be internalized by targeted cells.This study aimed to investigate the possibility of using Na131I labeled LyP-1 peptide as an imaging agent or a therapeutic radiopharmaceutical in breast carcinoma and its metastasis.Methods The 10-mer cyclic peptide contained the LyP-1 sequence (YCGNKRTRGC) was synthesized by the solid phase method.Disulfide bonds between the cysteines maintain the cyclic structure.The LyP-1 peptide was labeled with Na131I using the chloramine-T method.The [131I] LyP-1 peptide and a [131I] control peptide were injected via tail vein into nude mice bearing MDA-MB-435 tumor xenografts.Biodistribution and imaging results in vivo were obtained.Results The labeling efficiencies of LyP-1 peptide reached 80％±5％ (n=5).The radiochemical purity was about 96％.The radiochemical purity of the labeled compound remains 92％ at 24 hours in human serum at 37℃.In the biodistribution studies,the [131I] LyP-1 peptide accumulated in the tumor to a higher level than in other organs.The [131I]LyP-1 peptide can successfully image the tumor in nude mice bearing MDA-MB-435 tumor xenografts.Conclusions The LyP-1 peptide could be effectively labeled with Na131I and the labeled compound is stable in human serum at 37℃ for 24 hours.The high specificity of [131I] LyP-1 peptide suggests it may be a promising new radiotracer for identifying tumors.关键词
LyP-1 peptide/MDA-MB-435 xenografis/biodistribution/imagingKey words
LyP-1 peptide/MDA-MB-435 xenografis/biodistribution/imaging引用本文复制引用
YU Ming-ming,WANG Rong-fu,CHEN Yue-hua,ZHOU Hai-zhong,DENG Xiao-hu..Radiolabeling LyP-1 peptide and preliminary biodistribution evaluation in mice bearing MDA-MB-435 xenografts[J].中华医学杂志（英文版）,2013,126(3):471-475,5.
