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Endodontic bacteria from primary and persistent endodontic lesions in Chinese patients as identified by cloning and 16S ribosomal DNA gene sequencing

LI Xin ZHU Xiao-fei ZHANG Cheng-fei Peter Cathro CJ Seneviratne SHEN Song

中华医学杂志（英文版）2013，Vol.126Issue(4)：634-639,6.

中华医学杂志（英文版）2013，Vol.126Issue(4)：634-639,6.DOI:10.3760/cma.j.issn.0366-6999.20122285

Endodontic bacteria from primary and persistent endodontic lesions in Chinese patients as identified by cloning and 16S ribosomal DNA gene sequencing

LI Xin ¹ZHU Xiao-fei ²ZHANG Cheng-fei ³Peter Cathro ⁴CJ Seneviratne ⁵SHEN Song⁶

作者信息

1. Faculty of Dentistry, Peking University Third Hospital, Beijing,100191, China
2. Department of Special,School and Hospital of Stomatology, Peking University, Beijing, 100081, China
3. Comprehensive Dental Care , Faculty of Dentistry, the University of Hong Kong, Hong Kong, China
4. Department of Endodontics, School of Dentistry University of Adelaide, Australia
5. Oral Biosciences , Faculty of Dentistry, the University of Hong Kong, Hong Kong, China
6. Department of Operative and Endodontic,School and Hospital of Stomatology, Peking University, Beijing, 100081, China
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摘要

Abstract

Background Few literatures pertain to the 16S ribosomal DNA (16S rDNA) analysis of bacteria contributing to primary and persistent endodontic lesions,with no information available for the Chinese population.As such,we investigated endodontic bacteria associated with primary and persistent endodontic lesions in adult Chinese patients living in Beijing,China using 16S rDNA gene sequencing techniques.Methods Endodontic microbial samples were obtained from fourteen adult Chinese patients and subjected to DNA extraction.Pllymerase chain reaction (PCR) products were cloned and 100 clones from each generated library were randomly selected.Purified plasmid DNA with 16S rDNA gene inserts was sequenced,and the sequences were searched against GenBank databases using the BLASTN algorithm.Only significant identification with the highest-scored BLAST result and 99％ minimum similarity was considered for phylotyping.Results More than 150 taxa were obtained.Primary endodontic infection was mainly associated with Burkholderia cepacia,Actinomyces,Aranicola spp.and Streptococcus sanguinis,whilst Burkholderia cepacia was predominant in the persistent endodontic infections.Conclusion There is a difference in the species profile associated with endodontic infections of Chinese patients living in Beiiing in comoarison to other geographical or ethnic reports.

关键词

16S ribosomal DNA/endodontic bacteria/persistent infections/primary infections

Key words

16S ribosomal DNA/endodontic bacteria/persistent infections/primary infections

引用本文复制引用

LI Xin,ZHU Xiao-fei,ZHANG Cheng-fei,Peter Cathro,CJ Seneviratne,SHEN Song..Endodontic bacteria from primary and persistent endodontic lesions in Chinese patients as identified by cloning and 16S ribosomal DNA gene sequencing[J].中华医学杂志（英文版）,2013,126(4):634-639,6.

基金项目

This study was supported by grants from the National Natural Science Foundation of China (Nos.30840091 and 81000428). （Nos.30840091 and 81000428）

中华医学杂志（英文版）

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ISSN：0366-6999

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