中华医学杂志（英文版）2013，Vol.126Issue(4)：716-721,6.DOI:10.3760/cma.j.issn.0366-6999.20122255
Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials
Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials
HUANG Chun-mei 1YU Lian-hui 1PU Cheng-wei 2WANG Xin 1WANG Geng 1SHEN Li-song 3WANG Jian-zhong2
作者信息
- 1. Department of Clinical Laboratory, Chinese Academy of Medical Sciences & Peking Union Medical College Hospital, Beijing 100730, China
- 2. Department of Clinical Laboratory, Peking University First Hospital, Beijing 100034, China
- 3. Department of Clinical Laboratory, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
- 折叠
摘要
Abstract
Background White blood cell (WBC) counts and differentials performed using an automated cell counter typically require manual microscopic review.However,this last step is time consuming and requires experienced personnel.We evaluated the clinical efficiency of using flow cytometry (FCM) employing a six-antibody/five-color reagent for verifying automated WBC differentials.Methods A total of 56 apparently healthy samples were assessed using a five-color flow cytometer to verify the normal reference ranges of WBC differentials.WBC differentials of 622 samples were also determined using both a cell counter and FCM.These results were then confirmed using manual microscopic methods.Results The probabilities for all of the parameters of WBC differentials exceeded the corresponding normal reference ranges by no more than 7.5％.The resulting WBC differentials were well correlated between FCM and the cell counter (r ＞0.88,P ＜0.001),except in the case of basophils.Neutrophils,lymphocytes,and eosinophils were well correlated between FCM and standard microscopic cytology assessment (r ＞0.80,P ＜0.001).The sensitivities of FCM for identification of immature granulocytes and blast cells (72.03％ and 22.22％,respectively) were higher than those of the cell counter method (44.92％ and 11.11％,respectively).The specificities of FCM were all above 85％,substantially better than those of the cell counter method.Conclusion These five-color FCM assays could be applied to accurately verify abnormal results of automated assessment of WBC differentials.关键词
flow cytometry/white blood cell count/hematology/manual microscopyKey words
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HUANG Chun-mei,YU Lian-hui,PU Cheng-wei,WANG Xin,WANG Geng,SHEN Li-song,WANG Jian-zhong..Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials[J].中华医学杂志（英文版）,2013,126(4):716-721,6.
