中山大学学报（自然科学版）2013，Vol.52Issue(1)：118-122,129,6.
β-葡萄糖苷酶高产菌株及其应用研究
Application Studies on High Activity β-Glucosidase Producing Strain
摘要
Abstract
Studies of β - glucosidase enzymatic properties from Hypocrea sp. W63 show that, enzyme activity determined by pNPG (4 - nitrophenyl β - D - glucopyranoside) can attain to 482. 1 U/mL. The optimum reaction pH value and temperature are 4. 8 and 65℃, respectively. 10% of ethanol has the greatest promotion, the β - glucosidase activity can nearly increased double and the ethanol tolerance of β -glucosidase can attain to 30%. When simultaneous saccharification and fermentation (SSF) carried out for 120 h with β- glucosidase adjunction, 41. 25 g/L of ethanol can be obtained. Compared with control, the ethanol production can increase nearly 2 - fold. The β- glucosidase with high enzyme activity , thermostable and alcohol tolerance for SSF application, indicate that the β- glucosidase producing strain has great potent application on cellulosic ethanol production.关键词
β-葡萄糖苷酶/耐高温/耐乙醇/同步糖化发酵Key words
β- glucosidase/ thermostable/ ethanol tolerance/ simultaneous saccharification and fermentation分类
生物科学引用本文复制引用
梁翠谊,许敬亮,袁振宏,张宇,徐惠娟,庄新姝,高月淑..β-葡萄糖苷酶高产菌株及其应用研究[J].中山大学学报（自然科学版）,2013,52(1):118-122,129,6.基金项目
国家科技支撑计划资助项目(2011BAD22B01) （2011BAD22B01）
国家自然科学基金资助项目(21176237,21211140237) （21176237,21211140237）
国家高新技术研究发展计划(863计划)资助项目(2013AA065803) （863计划）
