安徽农业科学2013，Vol.41Issue(4)：1435-1437,3.
水稻SDG711蛋白C末端原核表达及多克隆抗体制备
Prokaryotic Expression and Polyclonal Antibody Preparation of SDG711 C-terminal from Rice
摘要
Abstract
[Objective] This study aimed to conduct prokaryotic expression of rice SDG711 C-terminal and prepare its polyclonal antibody. [ Method] C-terminal of rice SDG711 containing relatively intensive antigen determinants was selected for prokaryotic expression, prokaryotic expression vector pET28a-711C was constructed and the recombinant plasmid was transformed into Escherkhia coli BL21 (DE3) competent cells. The recombinant fusion protein was induced by IPTG and purified to immunize a New Zealand white rabbit as the antigen, and polyclonal antibody was obtained for Western-blot analysis. [Result] The polyclonal antibody prepared could efficiently detect the antigen expression. [Conclusion] This study laid the foundation for further investigating the functions of SDG711 protein.关键词
水稻/SDG723/原核表达/多克隆抗体Key words
Rice/ SDG711/ Prokaryotic expression/ Polyclonal antibody分类
农业科技引用本文复制引用
张志刚,李超,林欣欣,巫光宏,杜平州,王玉琪..水稻SDG711蛋白C末端原核表达及多克隆抗体制备[J].安徽农业科学,2013,41(4):1435-1437,3.基金项目
广东省高等学校科技创新项目(2012KJCX0021) （2012KJCX0021）
国家自然科学基金(30700052). （30700052）
